A design strategy to generate a SARS-CoV-2 RBD vaccine that abrogates ACE2 binding and improves neutralizing antibody responses. Ratswohl C, Vázquez García C, Ahmad AUW, Gonschior H, Lebedin M, Silvis CE, Spatt L, Gerhard C, Lehmann M, Sander LE, Kurth F, Olsson S, de la Rosa K. Eur J Immunol. 2023 Oct;53(10):e2350408. doi: 10.1002/eji.202350408. Epub 2023 Jul 23. PMID: 37435628. Link
Discriminating promiscuous from target-specific autoantibodies in COVID-19. Lebedin M, García CV, Spatt L, Ratswohl C, Thibeault C, Ostendorf L, Alexander T, Paul F, Sander LE, Kurth F, de la Rosa K. Eur J Immunol. 2023 May;53(5):e2250210. doi: 10.1002/eji.202250210. Epub 2023 Apr 3. PMID: 36856018. Link
Application of a Spacer-nick Gene-targeting Approach to Repair Disease-causing Mutations with Increased Safety. Tran NT, Lebedin M, Danner E, Kühn R, Rajewsky K, Chu VT. Bio Protoc. 2023 Apr 20;13(8):e4661. doi: 10.21769/BioProtoc.4661. PMID: 37113334; PMCID: PMC10127048. Link
Different classes of genomic inserts contribute to human antibody diversity. Lebedin M, Foglierini M, Khorkova S, Vázquez García C, Ratswohl C, Davydov AN, Turchaninova MA, Daubenberger C, Chudakov DM, Lanzavecchia A, de la Rosa K. Proc Natl Acad Sci U S A. 2022 Sep 6;119(36):e2205470119. doi: 10.1073/pnas.2205470119. Epub 2022 Aug 29. PMID: 36037353; PMCID: PMC9457163.Link
SARS-CoV-2 mRNA vaccinations fail to elicit humoral and cellular immune responses in patients with multiple sclerosis receiving fingolimod. Meyer-Arndt L, Braun J, Fauchere F, Vanshylla K, Loyal L, Henze L, Kruse B, Dingeldey M, Jürchott K, Mangold M, Maraj A, Braginets A, Böttcher C, Nitsche A, de la Rosa K, Ratswohl C, Sawitzki B, Holenya P, Reimer U, Sander LE, Klein F, Paul F, Bellmann-Strobl J, Thiel A, Giesecke-Thiel C. J Neurol Neurosurg Psychiatry. 2022 Sep;93(9):960-971. doi: 10.1136/jnnp-2022-329395. Epub 2022 Jul 14. PMID: 35835468; PMCID: PMC9380499. Link
Precise CRISPR-Cas-mediated gene repair with minimal off-target and unintended on-target mutations in human hematopoietic stem cells. Tran NT, Danner E, Li X, Graf R, Lebedin M, de la Rosa K, Kühn R, Rajewsky K, Chu VT. Sci Adv. 2022 Jun 3;8(22):eabm9106. doi: 10.1126/sciadv.abm9106. Epub 2022 Jun 3. PMID: 35658035; PMCID: PMC9166625.Link
A Barcoded Flow Cytometric Assay to Explore the Antibody Responses Against SARS-CoV-2 Spike and Its Variants. Vesper N, Ortiz Y, Bartels-Burgahn F, Yang J, de la Rosa K, Tenbusch M, Schulz S, Finzel S, Jäck HM, Eibel H, Voll RE, Reth M. Front Immunol. 2021 Sep 23;12:730766. doi: 10.3389/fimmu.2021.730766. PMID: 34630410; PMCID: PMC8496935. Link
A Public Health Antibody Screening Indicates a 6-Fold Higher SARS-CoV-2 Exposure Rate than Reported Cases in Children. Hippich M, Holthaus L, Assfalg R, Zapardiel-Gonzalo J, Kapfelsperger H, Heigermoser M, Haupt F, Ewald DA, Welzhofer TC, Marcus BA, Heck S, Koelln A, Stock J, Voss F, Secchi M, Piemonti L, de la Rosa K, Protzer U, Boehmer M, Achenbach P, Lampasona V, Bonifacio E, Ziegler AG. Med (N Y). 2021 Feb 12;2(2):149-163.e4. doi: 10.1016/j.medj.2020.10.003. Epub 2020 Oct 29. PMID: 33163984; PMCID: PMC7598360. Link
A Homology Independent Sequence Replacement Strategy in Human Cells Using a CRISPR Nuclease. Danner E, Lebedin M, de la Rosa K, Kühn R. Open Biology. 2021 Jan. 200283. Link
A Barcoded Flow Cytometric Assay to Explore the Antibody Responses Against SARS-CoV-2 Spike and Its Variants. Vesper N, Ortiz Y, Bartels-Burgahn F, Yang J, de la Rosa K, Tenbusch M, Schulz S, Finzel S, Jäck HM, Eibel H, Voll RE, Reth M. Front Immunol. 2021 Sep 23;12:730766. doi: 10.3389/fimmu.2021.730766. eCollection 2021.Link
A public health antibody screening indicates a 6-fold higher SARS-CoV-2 exposure rate than reported cases in children. Hippich M, Holthaus L, Assfalg R, Zapardiel-Gonzalo J, Kapfelsperger H, Heigermoser M, Haupt F, Ewald DA, Welzhofer TC, Marcus BA, Heck S, Koelln A, Stock J, Voss F, Secchi M, Piemonti L, de la Rosa K, Protzer U, Boehmer M, Achenbach P, Lampasona V, Bonifacio E, Ziegler AG. Med. 2021 Feb; 149-163. e4.Link
Incomplete genetic reconstitution of B cell pools contributes to prolonged immunosuppression after measles. Petrova VN, Sawatsky B, Han AX, Laksono BM, Walz L, Parker E, Pieper K, Anderson CA, de Vries RD, Lanzavecchia A, Kellam P, von Messling V, de Swart RL, Russell CA. Sci Immunol. 2019 Nov 1;4(41).Link
Public antibodies to malaria antigens generated by two LAIR1 insertion modalities. Pieper K*, Tan J*, Piccoli L*, Foglierini M, Barbieri S, Chen Y, Silacci-Fregni C, Wolf T, Jarrossay D, Anderle M, Abdi A, Ndungu FM, Doumbo OK, Traore B, Tran TM, Jongo S, Zenklusen I, Crompton PD, Daubenberger C, Bull PC, Sallusto F, Lanzavecchia A. Nature. 2017. Aug 31;548(7669):597-601.Link
A LAIR1 insertion generates broadly reactive antibodies against malaria variant antigens. Tan J*, Pieper K*, Piccoli L*, Abdi A, Foglierini M, Geiger R, Tully CM, Jarrossay D, Ndungu FM, Wambua J, Bejon P, Fregni CS, Fernandez-Rodriguez B, Barbieri S, Bianchi S, Marsh K, Thathy V, Corti D, Sallusto F, Bull P, Lanzavecchia A. Nature. 2016 Jan 7; 529(7584):105-9.Link
Disturbed B-lymphocytes selection in autoimmune lymphoproliferative syndrome. Janda A, Schwarz K, van der Burg M, Vach W, Ijspeert H, Lorenz MR, Elgizouli M, Pieper K, Fisch P, Hagel J, Lorenzetti R, Seidl M, Roesler J, Hauck F, Traggiai E, Speckmann C, Rensing-Ehl A, Ehl S, Eibel H, Rizzi M. Blood. 2016 May 5;127(18):2193-202. Link
Deficiency of the B cell-activating factor receptor results in limited CD169+ macrophage function during viral infection. Xu HC, Huang J, Khairnar V, Duhan V, Pandyra AA, Grusdat M, Shinde P, McIlwain DR, Maney SK, Gommerman J, Löhning M, Ohashi PS, Mak TW, Pieper K, Sic H, Speletas M, Eibel H, Ware CF, Tumanov AV, Kruglov AA, Nedospasov SA, Häussinger D, Recher M, Lang KS, Lang PA. J Virol. 2015 May;89(9):4748-59.Link
A common single nucleotide polymorphism impairs B-cell activating factor receptor's multimerization, contributing to common variable immunodeficiency. Pieper K, Rizzi M, Speletas M, Smulski CR, Sic H, Kraus H, Salzer U, Fiala GJ, Schamel WW, Lougaris V, Plebani A, Hammarstrom L, Recher M, Germenis AE, Grimbacher B, Warnatz K, Rolink AG, Schneider P, Notarangelo LD, Eibel H. J Allergy Clin Immunol. 2014 Apr;133(4):1222-5. Link
Sphingosine-1-phosphate receptors control B-cell migration through signaling components associated with primary immunodeficiencies, chronic lymphocytic leukemia, and multiple sclerosis. Sic H, Kraus H, Madl J, Flittner KA, von Münchow AL, Pieper K, Rizzi M, Kienzler AK, Ayata K, Rauer S, Kleuser B, Salzer U, Burger M, Zirlik K, Lougaris V, Plebani A, Römer W, Loeffler C, Scaramuzza S, Villa A, Noguchi E, Grimbacher B, Eibel H. J Allergy Clin Immunol. 2014 Aug;134(2):420-8.Link
A feeder-free differentiation system identifies autonomously proliferating B cell precursors in human bone marrow. Kraus H, Kaiser S, Aumann K, Bönelt P, Salzer U, Vestweber D, Erlacher M, Kunze M, Burger M, Pieper K, Sic H, Rolink A, Eibel H, Rizzi M. J Immunol. 2014 Feb 1;192(3):1044-54.Link
B-cell biology and development. Pieper K, Grimbacher B, Eibel H. J Allergy Clin Immunol. 2013 Apr;131(4):959-71.Link
* authors contributed equally
