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The core provides users access to quality-controlled pluripotent stem cells. This ranges from reference hiPSC  lines from healthy donors that are available cryopreserved and ready ‘in-culture’. In addition to the reference lines, users can request frozen vials of non-standard lines.

All lines are registered in hPSCreg and robustly validated and quality controlled. The lines were generated form healthy donor samples and produce high differentiation efficiencies in the number of various protocols. For the full portfolio of cell lines see tables below.

Reference hiPSC lines (healthy donor)

The reference lines are available to all researchers at BIH, Charité and MDC. Cells can be requested via openIRIS.

All external users are required to execute a Material Transfer Agreement (MTA) with us.

reference cell lines
Cell Line name Gender Cell type of origin Reprogramming method  
BIHi001-B male Fibroblast Of Dermis single-stranded RNA replicon vector  
BIHi004-B female Fibroblast Of Dermis Episomal  
BIHi005-A male Fibroblast Of Dermis mRNA  
BIHi250-A female Fibroblast Of Dermis single-stranded RNA replicon vector  

Allen cell lines

The Allen Cell Catalog contains high quality, certified fluorescently tagged hiPSC lines that target key cellular structures and substructures.

CUSCO has filed an MTA that permits all researchers at BIH, Charité and MDC to work with the hiPSC lines of the Allen Cell Catalog provided through the core.

Currently available lines (quality controlled banks available)

Allen cell lines
Line name Tagged Gene Structure Fluorescent Tag
UCSFi001-A untagged parental line mone none
UCSFi001-A-1 Paxillin (PXN) Matrix adhesions EGFP
UCSFi001-A-8 SEC61 translocon beta subunit (SEC61B) Endoplasmic reticulum mEGFP
UCSFi001-A-5 translocase of outer mitochondrial membrane 20 (TOMM20) mitochondria mEGFP
UCSFi001-A-2 tubulin-alpha 1b (TUBA 1B) microtubules mEGFP
UCSFi001-A-6 lamin B1 (LMNB1) Nuclear envelope mEGFP
UCSFi001-A-9 Fibrillarin (FBL) nucleolus (dense fibrillar compontent) mEGFP
UCSFi001-A-3 actin beta (ACTB) Actin filaments mEGFP
UCSFi001-A-7 Desmoplakin (DSP) desmosomes mEGFP
UCSFi001-A-13 lysosomal associated membrane protein 1 (LAMP1) lysosomes mEGFP
UCSFi001-A-10 tight junction protein 1 (TJP1) Tight junctions mEGFP
UCSFi001-A-11 myosin heavy chain 10 (MYH10) Actomyosin bundles mEGFP
UCSFi001-A-14 ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1) Golgi mEGFP
UCSFi001-A-15 ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1) Golgi mEGFP
UCSFi001-A-18 microtubule associated protein 1 light chain 3 beta (MAP1LC3B) autophagosomes mEGFP
UCSFi001-A-16 tubulin-alpha 1b (TUBA1B) microtubules mTagRFP-T
UCSFi001-A-17 centrin 2 (CETN2) centrioles mTagRFP-T
UCSFi001-A-19 solute carrier family 25 member 17 (SLC25A17) peroxisomes mEGFP
UCSFi001-A-12 safe harbor locus (AAVS1) cytoplasm mEGFP
UCSFi001-A-36 safe harbor locus (AAVS1) cytoplasm mEGFP
UCSFi001-A-26 troponin I1, slow skeletal type (TNNI1) Sacromeric thin filaments mEGFP
UCSFi001-A-20 RAB5A, member RAS oncogene family (RAB5A) endosomes mEGFP
UCSFi001-A-21 RAB5A, member RAS oncogene family (RAB5A) endosomes mEGFP
UCSFi001-A-30 ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 (ATP2A2) Sarcoplasmic reticulum/endoplasmic reticulum mEGFP
UCSFi001-A-27 Titin (TTN) Sacromeres (M-line tag) mEGFP
UCSFi001-A-29 myosin light chain 7 (MYL7) Sacromeres thick filaments mEGFP
UCSFi001-A-22 gap junction protein alpha 1 (GJA1) Gap junctions mEGFP
UCSFi001-A-23 safe harbor locus (AAVS1) Plasma membrane mTagRFP-T
UCSFi001-A-24 nucleophosmin 1 (NPM1) Nucleolus (granular component) mEGFP
UCSFi001-A-25 catenin beta 1 (CTNNB1) Adherens junctions mEGFP
UCSFi001-A-33 Sec61 translocon beta subunit / lamin B1 (SEC61B/LMNB1) endoplasmic reticulum/ nuclear envelope mEGFP / mTagRFP-T
UCSFi001-A-32 myosin light chain 2 (MYL2) Sacromeres thick filaments mEGFP
UCSFi001-A-28 histone cluster 1 H2B family member j (HIST1H2BJ) histones mEGFP
UCSFi001-A-34 Dystrophin (DMD) costameres mEGFP
UCSFi001-A-31 nucleoporin 153 (NUP153) Nuclear pores mEGFP
UCSFi001-A-37 SRY-box transcription factor 2 (SOX2) Transcription factor mEGFP
UCSFi001-A-4 actinin alpha 2 (ACTN2) Sarcomeric z-disks mEGFP
UCSFi001-A-35 FUS RNA binding protein (FUS) Paraspeckles / stress granules mEGFP
UCSFi001-A-38 fibrillarin / nucleophosmin 1 (FBL / NPM1) Nucleolus (dense fibrillary component / granular component) mEGFP / mTagRFP-T
UCSFi001-A-55 translocase of outer mitochondrial membrane 20 / tubulin-alpha 1b (TOMM20 / TUBA1B) Mitochondria / microtubules mEGFP / mTagRFP-T

Patient derived/disease specific hiPSC lines

CUSCO has maintains a growing portfolio of patient derived/disease specific hiPSC lines. If you are interested to work with these please get in contact with us.

available lines:
Line name Disease Gender Cell type of origin
BIHi006-D Focal segmental glomerulosclerosis (FSGS) male PBMC
BIHi007-A Focal segmental glomerulosclerosis (FSGS) male PBMC
BIHi008-A Focal segmental glomerulosclerosis (FSGS) female PBMC
BIHi009-A Focal segmental glomerulosclerosis (FSGS) male PBMC
BIHi010-A Focal segmental glomerulosclerosis (FSGS) male PBMC
BIHi011-A Focal segmental glomerulosclerosis (FSGS) female PBMC
BIHi012-A Focal segmental glomerulosclerosis (FSGS) female PBMC
BIHi015-A Focal segmental glomerulosclerosis (FSGS) male PBMC
BIHi016-A Focal segmental glomerulosclerosis (FSGS) female PBMC
BIHi017-A Focal segmental glomerulosclerosis (FSGS) male PBMC
BIHi018-A Focal segmental glomerulosclerosis (FSGS) male PBMC
BIHi019-A Focal segmental glomerulosclerosis (FSGS) male PBMC
BIHi024-A Focal segmental glomerulosclerosis (FSGS) female PBMC
BIHi025-A Focal segmental glomerulosclerosis (FSGS) male PBMC
BIHi028-B Focal segmental glomerulosclerosis (FSGS) female PBMC
BIHi029-A Focal segmental glomerulosclerosis (FSGS) male PBMC
BIHi030-C Focal segmental glomerulosclerosis (FSGS) male PBMC
BIHi031-B Focal segmental glomerulosclerosis (FSGS) female PBMC
BIHi032-A Focal segmental glomerulosclerosis (FSGS) male PBMC
BIHi038-B Focal segmental glomerulosclerosis (FSGS) female PBMC
BCRTi001-A fibrodysplasia ossificans progressive (FOP) male Urine
BCRTi002-A fibrodysplasia ossificans progressive (FOP) female Urine

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