BIH Core Facility Stem Cells
Pluripotent stem cells are unique tools for closing the gap between the identification of disease-causing molecules or cellular mechanisms and the development of suitable therapeutic approaches. Specifically the development of patient specific disease models will be of major importance.
The mission of the BIH Core Facility Stem Cells is to support basic and translational research by facilitating all aspects of human induced pluripotent stem cell (hiPSC) technology including the derivation, differentiation and distribution of humaniPS cell lines. Additionally, the facility will provide scientists with state of the art protocols and techniques for proper handling and manipulation of human pluripotent stem cells. Furthermore, the Core Facility will organize several hands-on training courses of standardized pluripotent stem cell culture techniques.
The facility has two locations to best serve the researchers at BIH, one at the Charité Campus Virchow-Klinikum (CVK, Wedding), and the other is located at the Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC, Berlin-Buch).
Location Charité (CVK)
Dr. Harald Stachelscheid (Facility Head)
Judit Küchler (Technical Assistant)
Kristin Fischer (Technical Assistant)
Janine Cernoch (Technical Assistant)
Claudia Schaar (Administration)
Dr. Sebastian Diecke (Facility Head)
Sandra Wegener (Technical Assistant)
Polixeni Burazi (Technical Assistant)
Norman Krüger (Technical Assistant)
Anna Iwanska (Technical Assistant)
Prof. Norbert Hübner*: Research group "Genetics and Genomics of Cardiovascular Diseases"
* current speaker
Prof. Georg Duda*: Director Julius Wolff Institute for Biomechanics and Musculoskeletal Regeneration, BCRT
The full spectrum of services provided by the core facility is currently under heavy development but will include the following:
- Provision of reference hiPSC lines
- Isolation, reprogramming, characterization
- Development and provision of standardized protocols including generation, culture and differentiation
- Project consultancy
- Data Management and banking
- Genome editing
- Cell labeling
The generation and differentiation, but also establishment of disease models, require a profound knowledge and hands-on expertise of maintaining and manipulating hiPSCs in tissue culture.
The processes involved in these aspects of hPSC handling in tissue culture are labor intensive and distinct from that of other mammalian cell cultures. The BIH Core Facility Stem Cells organizes regular training on these methodologies.
The training is intended for researchers and technicians who are planning projects using human iPSCs and/or will start working with hiPSCs in the near future.
The next training session will be from 31 May to 2 June 2017.