Core Facility Stem Cells

Pluripotent stem cells are unique tools for closing the gap between the identification of disease-causing molecules or cellular mechanisms and the development of suitable therapeutic approaches. Specifically the development of patient specific disease models will be of major importance. 

The mission of the BIH Core Facility Stem Cells is to support basic and translational research by facilitating all aspects of human induced pluripotent stem cell (hiPSC) technology including the derivation, differentiation and distribution of humaniPS cell lines. Additionally, the facility will provide scientists with state of the art protocols and techniques for proper handling and manipulation of human pluripotent stem cells. Furthermore, the Core Facility will organize several hands-on training courses of standardized pluripotent stem cell culture techniques.

The facility has two locations to best serve the researchers at BIH, one at the Charité Campus Virchow-Klinikum (CVK, Wedding), and the other is located at the Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC, Berlin-Buch).

Services

Isolation of primary cells form patient samples

e.g. skin, blood, urine

Reprogramming of cells into induced pluripotent stem cells

application of non integrating vectors e.g. Sendai Virus, mRNA

Characterization and Quality Control of hPSC

- Expression of pluripotency markers (FACS, IF, PCR)
- Whole Genome Gene Expression Profiling (PluriTest) 
- Differentiation potential (differentiation into cells of the three germ layers, 
   teratoma formation)
- genetic integrity (SNP Array, KaryoBoB)
- Identity (STR Fingerprinting)
- microbial contamination (sterility, mykoplasma, human viral pathoges)

Gene editing and cell labeling

Gene KnockOut, KnockIn of transgenes e.g. GFP, introduction and correction of mutations using CRISPR/Cas or TALEN 

Provision of hiPSC reference lines and banking

- production and validation of cell banks
- the Core Facility established a range of fully characterized hiPSC lines (e.g. BIHi001-A) which can be used as a reference
- an overview of the provided cell lines can be found in the human Pluripotent Stem Cell Registry (hPSCreg).

Establishment of differentiation protocols and provision of hiPSC derived differentiated cells

e.g. cardiomyocytes, endothelial cells, neurons, neural stem cells, renal progenitor cells, hepatocytes

Organoids

e.g. cerebral organoids, kidney organoids

Provision of infrastructure und equipment

FACS (MACSQuant Analyzer VYB and MACSQuant Sorter Tyto), RT-PCR (QuantStudio 6), Phase-contrast-, stereo- and fluorescence-microscopes, transfection (Amaxa, Neon), hypoxia-incubators, Picking Hoods

Provision of reagents

e.g. special media, viral/non-viral vectors (in cooperation with the Viral Core Facility of the Charité)

Provision of standardized protocols

the core provides it users a constantly growing portfolio of state of the art protocols that includes methods for culture, characterization and quality control, cryo-preservation, differentiation and genetic manipulation.

Project consultancy

support for project planning, grand application and ethical approvals

Training Opportunities

The generation and differentiation, but also establishment of disease models, require a profound knowledge and hands-on expertise of maintaining and manipulating hiPSCs in tissue culture.

The processes involved in these aspects of hPSC handling in tissue culture are labor intensive and distinct from that of other mammalian cell cultures. The BIH Core Facility Stem Cells organizes regular training on these methodologies.

The training is intended for researchers and technicians who are planning projects using human iPSCs and/or will start working with hiPSCs in the near future.

The next training session will be from 17 - 19 October 2018.

For a project-related support we would like to ask you to fill out the service request form.

For booking our device and project management we use the plattform Open IRIS (location Charitélocation MDC).

Team

Location Charité (CVK)

Location MDC

Dr. Harald Stachelscheid (Facility leader)

Dr. Sebastian Diecke (Facility leader)

Judit Küchler (Technical assistent)

Narasimha Swami Telugu (research assistent)

Kristin Fischer (Technical assistent)

Norman Krüger(Technical assistent)

Janine Cernoch (Technical assistent)

Anna Iwanska (Technical assistent)

Tanja Fisch (Technical assistent)

Silke Kurths (Technical assistent)

Claudia Schaar (Administration)

Sandra Schommer-Leitner (Technical assistent)

 

Carolin Genehr (Technical assistent)

 

Steering Committee

MDC

Charité

Prof. Norbert Hübner*: Research group "Genetics and Genomics of Cardiovascular Diseases"

Prof. Georg Duda*: Director Julius Wolff Institute for Biomechanics and Musculoskeletal Regeneration, BCRT

Dr. Ralf Kühn: Research group "iPS cell based disease modeling"

Prof. Petra Reinke: Nephrology, Transplantation medicine, BCRT

Prof. Ana Pombo: Research group "Epigenetic regulation and chromatin architecture"

Prof. Ulf Landmesser: BIH professorship for cardiology

Prof. Thomas Willnow: Research group "Molecular Cardiovascular Research"

Prof. Christian Rosenmund: Neuroscience with the focus on cellulatr and molecular Neurobiology, spokesperson NeuroCure

 * current speaker

Prof. Clemens Schmitt: Tumor genetics and cellular stress responses