The QUEST 1,000 € NULL Results And Replication Study Award
Publish your NULL results – Fight the negative publication bias!
Publish your Replication Study – Fight the replication crises!
Traditionally there is a strong bias against results that did not confirm or support the hypothesis researchers set out to test. Such results are called NULL results (as the Null hypothesis was not rejected), and rarely get published (‘file drawer phenomenon’) and are therefore lost to the scientific community. NULL results of well performed studies are important for many reasons. Due to the current bias towards ‘positive results’ aggregation of the available literature in systematic reviews overestimate the efficacy of drugs or effect sizes of biological phenomena. Knowing about ‘blind alleys’ and ‘dead ends’ may prevent others from repeating experiments unnecessarily. And surprising to many, NULL results are more informative than ‘positive’ results. Their positive and negative predictive values are much higher than those of results that support our hypotheses (for details see here, and here).
Until a decade ago or so it was almost impossible to publish NULL results, unless they refuted a spectacular claim. Fortunately, this has changed, and numerous journals now publish results of sound studies regardless of their outcome (negative, inconclusive), such as F1000Res, PeerJ, PlosONE and many more. Incidentally, all these journals publish Open Access (see also our Open Access Publication Fund).
Reproducibility is fundamental to scientific Progress (see also Statement of the German Research Foundation, only in German). Unfortunately, the replication of studies carries little prestige in academic research. Only recently have systematic replication studies demonstrated that current biomedicine has a serious replication problem, which led to the declaration of a reproducibility crisis. We need more systematic replication efforts of high quality, regardless of their outcome.
QUEST is giving away these awards of 1,000 € to first/last/corresponding authors (BIH or Charité affiliation) of preclinical or clinical research papers in which the main result is a NULL or ‘negative’ or in which the replication of own results or the results of others is attempted. Papers must have been published 2017 onwards.
The award (travel costs or consumables) will be administered through Charité. Prizes can be spent until the end of 2021. Awards are given on an ongoing basis. Awardees will be featured on the QUEST web pages.
To claim your award, send an email with a short statement and the publication to email@example.com.
Publications of authors (independent of the author´s position) which are also QUEST employees cannot be considered.
Each researcher may apply to every call only once.
QUEST NULL results and Replication study Award
- The publication "Analysis of Lymphocytic DNA Damage in Early Multiple Sclerosis by Automated Gamma-H2AX and 53BP1 Foci Detection: A Case Control Study" receives a QUEST NULL results Award for reporting null results as major findings in a clinical case-control study. The article was published 2016 in PloS One (DOI: 10.1371/journal.pone.0147968). Applicant: Dr. Ludwig Rasche, Department of Neurology and NeuroCure Clinical Research Center, Charité – Universitätsmedizin Berlin.
- The publication "Individual Alpha Frequency Relates to the Sound-Induced Flash Illusion" receives a QUEST Replication Study award. The study successfully reproduces and extends the findings of a 2015 published study. The replication of the previous results increases the robustness of observations that describe an important neuronal mechanism. The article was published 2017 in Multisensory Research (DOI: 10.1163/22134808-00002572). Applicant: Dr. Julian Keil, Klinik für Psychiatrie und Psychotherapie der Charité im St. Hedwig-Krankenhaus Berlin.
- The publication "Normal volumes and microstructural integrity of deep gray matter structures in AQP41 NMOSD", a case-control study, receives a QUEST NULL results award. Against the background of conflicting previous findings, this article reports no involvement of deep gray matter in patients with NMOSD, an autoimmune CNS condition. The article was published 2016 in Neurology: Neuroimmunology & Neuroinflammation (DOI: 10.1212/NXI.0000000000000229). Applicant: Dr. Carsten Finke, Klinik für Neurologie, Charité – Universitätsmedizin Berlin.
- The publication "Investigation of hippocampal synaptic transmission and plasticity in mice deficient in the actinbinding protein Drebrin" - a preclinical, electrophysiological study in mice - receives a QUEST NULL results award. The study reports no differences between models as major findings. The results seem to contradict previous findings from other in vitro, ex vivo and in vivo experiments, as the authors point out. The article was published 2017 in Scientific Reports (DOI: 10.1038/srep42652). Applicant: Prof. Britta Eickholt, Institute of Biochemistry and NeuroCure-Cluster of Excellence, Charité – Universitätsmedizin Berlin.
- The publication "Hypertension and Risk of Post-Operative Cognitive Dysfunction (POCD): A Systematic Review and Meta-Analysis" receives a QUEST NULL results award. Based on a systematic data synthesis of the existing literature, the publication reports no higher risk for post-operative cognitive dysfuntion in patients with hypertension compared to patients without. The article was published 2017 in Clinical Practice & Epidemiology in Mental Health (DOI: 10.2174/1745017901713010027). Applicant: Dr. Insa Feinkohl, Molecular Epidemiology Group, Max-Delbrueck-Centrum for Molecular Medicin (MDC) in the Helmholtz Association.
- The publication "S1P receptor antagonists fingolimod and siponimod do not improve the outcome of experimental autoimmune myasthenia gravis mice after disease onset" receives a QUEST NULL results Award. The authors showed that the observation of an effect of an active agent on the onset of an autoimmune disease, did not translate to the treatment of the disease. While previous findings from preclinical studies indicated that sphingosine-1-phosphate (S1P) antagonists prevent the onset of autoimmune myasthenia gravis in mice, this publication, however, reports no effect on the course of the disease in a similar animal model. The article was published 2017 in the European Journal of Immunology (DOI:10.1002/eji.201747187). Applicant: Andreas Pelz, Department of Experimental Neurology and Department of Rheumatology and Clinical Immunology, Charité – Universitätsmedizin Berlin.
- The publication "Inflammatory-linked changes in CpG island methylation of three opioid peptide genes in a rat model for pain" receives a QUEST NULL results Award. Earlier studies indicated an upregulation of opiod genes in the context of peripheral inflammation. The authors report that a transcriptional regulation via methylation doesn`t seem to be a key regulator of opioid gene activation in immune cells during peripheral tissue inflammation in a rat model. The article was published 2018 in PLOS ONE (DOI:10.1371/journal.pone.0191698). Applicant: Prof. Christoph Stein, Klinik für Anästhesiologie und operative Intensivmedizin, Charité – Universitätsmedizin Berlin.
- The publication "Oral administration of a novel lipophilic PPAR δ agonist is not neuroprotective after rodent cerebral ischemia" receives a QUEST NULL results Award. In contradiction to previously published work on the role of PPARδ agonists in models of cerebral ischemia, the authors found no biologically relevant neuroprotective effect of a treatment with a PPARδ agonist. The publication contributes to the overall evaluation of the efficacy of PPARδ agonists as a neuroprotective therapy of ischemic stroke. The article was published 2018 in the Journal of Cerebral Blood Flow & Metabolism (DOI: 10.1177/0271678X17743876). Applicant: Dr. Samuel Knauss, Department of Experimental Neurology, Charité – Universitätsmedizin Berlin.
- The publication "The dexamethasone corticotropin releasing hormone test in healthy and depressed women with and without childhood adversity" receives a QUEST NULL results Award. In this controlled clinical study, the authors investigated the effects of childhood adverse events and depression on HPA axis activity. Contrasting previous findings, they did not find evidence supporting the assumption of distinct neuroendicrine endophenotypes in patients with major depressive disorder with and without childhood adverse experiences compared to healthy controls. The publication challenges previous assumptions and emphasizes the importance of further systematic disentanglement of factors that potentially contribute to altered HPA axis function. The article was published 2018 in Psychoneuroendocrinology (DOI: 10.1016/j.psyneuen.2017.10.016). Applicant: Prof. Katja Wingenfeld, Klinik für Psychiatrie und Psychotherapie, Campus Benjamin Franklin, Charité – Universitätsmedizin Berlin.
- The publication "A critical reexamination of doing arithmetic nonconsciously" receives a Replication Study award. By reanalyzing an already published data set, the authors were able to show that previous claims about the ability to solve equations without consciously perceiving them, are not fully supported. The publication emphasizes the importance of cumulative research strategies to provide evidence that allows valid and strong conclusions. The article was published 2017 in Psychonomic Bulletin & Review (DOI: 10.3758/s13423-017-1292-x). Applicant: PD Dr. Guido Hesselmann, Visual Perception Laboratory, Department of Psychiatry & Psychotherapy, Charité – Universitätsmedizin Berlin, Charité Campus Mitte.
- The publication "Octreotide Does Not Inhibit Proliferation in Five Neuroendocrine Tumor Cell Lines" receives a QUEST NULL results Award. The authors show that five established neuroendocrine tumor (NET) cell line models did not express tumor-like somatostatin receptors (SSTR) and no impact of somatostatin analogs (SSA) treatment alone was found. These results The octreotide resistance in examined cell line models underscores the importance of more physiologic tumor model systems. The article was published 2018 in Frontiers in Endocrinology (DOI: 10.3389/fendo.2018.00146). Applicant: Dr. Carsten Grötzinger, Med. Klinik m.S. Hepatologie und Gastroenterologie, Charité – Universitätsmedizin Berlin.
- The publication "Fingolimod therapy is not effective in a mouse model of spontaneous autoimmune peripheral polyneuropathy" receives a QUEST NULL results Award. This preclinical study showed that therapy of autoimmune polyneuropathy with fingolimod did not show any improvements regarding the disease progression or motor deficits compared to a vehicle treatment. This publication corroborates findings from a recent study in chronic inflammatory demyelinating polyradiculoneuropathy patients. The article was published 2018 in Scientific Reports (DOI: 10.1038/s41598-018-23949-4). Applicant: Dr. med. Petra Hühnchen, Klinik und Hochschulambulanz für Neurologie, Charité – Universitätsmedizin Berlin.
- The publication "General, crystallized and fluid intelligence are not associated with functional global network efficiency: A replication study with the human connectome project 1200 data set" receives a Replication Study award. Making use of data from the Human Connectome Project, this study set out to replicate earlier findings that general intelligence is associated with global functional network efficiency. These results could, however, not be reproduced, indicating that earlier reports, based on much smaller samples, might not be robust. The article was published 2018 in NeuroImage (DOI: 10.1016/j.neuroimage.2018.01.018). Applicant: Lea Waller, Department of Psychiatry and Psychotherapy, Charité – Universitätsmedizin Berlin.
- The publication "Liver-secreted RBP4 does not impair glucose homeostasis in mice" receives a QUEST NULL results Award. Based on the literature, the authors hypothesized that higher levels of RBP4, a major transport protein in blood, would impair glucose homeostasis in mice. However, elevating the expression of RBP4 had no such effect for both regular and high-fat diets, indicating that RBP4 does not impair glucose homeostasis. The article was published in 2018 in Journal of Biological Chemistry (DOI: 10.1074/jbc.RA118.004294). Applicant: Prof. Dr. Michael Schupp, Institute of Pharmacology, Charité - Universitätsmedizin Berlin.
- The publication "Impact of Intraoperative Hyperglycemia on Brain Structures and Volumes" receives a QUEST NULL results Award. Postmortem studies in septic patients indicated that hyperglycemic patients were more prone to increased rate of apoptosis of microglial cells compared to patients without hyperglycemia. In this prospective, observational study, however, the authors observed no effect of intraoperative hyperglycemia on postoperative brain structures and volumes in elderly patients undergoing major surgery. The article was published in 2018 in Journal of Neuroimaging (DOI: 10.1111/jon.12583). Applicant: Dr. med. Lachmann, Klinik für Anästhesiologie m.S. operative Intensivmedizin, Campus Virchow-Klinikum, Charité - Universitaetsmedizin Berlin.
- The publication "Effects of transcranial direct current stimulation on the cognitive control of negative stimuli in borderline personality disorder" receives a QUEST NULL results Award. This clinical study reports results from excitatory prefrontal stimulation in patients with borderline personality disorder, which lead to rejection of the hypothesis. Furthermore, hypotheses, sample size, exclusion criteria, and statistical analyses were pre-registered. The pre-registration, full data set, syntax, and statistical results are available at osf.io/g43bh/. The article was published in 2018 in Scientific Reports (DOI: 10.1038/s41598-018-37315-x). Applicants: Prof. Roepke and Dr. Schulze, Klinik für Psychiatrie und Psychotherapie, Campus Benjamin Franklin, Charité – Universitätsmedizin Berlin.
- The publication "Cardiac 123I-MIBG Scintigraphy in Neurodegenerative Parkinson Syndromes: Performance and Pitfalls in Clinical Practice" receives a QUEST NULL results Award. The authors show that even if 123I-MIBG scintigraphy is a known specific and valuable technique in scientific approaches and well-defined and highly selected samples, predictability of 123I-MIBG based nuclear medical diagnosis for individual cases and thus, feasibility in routine clinical practice is limited. The clinical series of the authors emphasize clinical verification of 123I-MIBG results on an individual basis in clinical routine. The article was published in 2019 in Frontiers in Neurology (DOI:10.3389/fneur.2019.00152). Applicant: Dr. Cornelia Skowronek, Movement Disorders and Neuromodulation Section, Charité – Universitätsmedizin Berlin.
- The publication "Long-Range Temporal Correlations in Resting State Beta Oscillations are Reduced in Schizophrenia" receives a QUEST Replication Study award. The authors could replicate the results of a previous study on long-range temporal correlations as a marker of network instability in people with schizophrenia. In their replication study, they used both a higher spatial resolution as well as stricter statistical methods compared to the original study. The article was published in 2019 in Frontiers in Psychiatry (DOI: 10.3389/fpsyt.2019.00517). Applicant: Dr. James Moran and Georgios Michail, Department of Psychiatry & Psychotherapy, Charité – Universitätsmedizin Berlin.
- The publication "No association between participant retention and the proportion of included elderly people in rheumatology trials: Results from a series of exploratory meta‐regression analyses" recieves a QUEST NULL results award. Based on a systematic data synthesis of the existing literature, the study found no association between participant retention and the proportion of included elderly people in rheumatology trials. This article was published 2019 in Arthritis Care & Research (DOI: 10.1002/acr.24051). Applicant: Andriko Palmowski, Department of Rheumatology and Clinical Immunology, Charité - Universitätsmedizin Berlin
- The publication "Physical Fitness Training in Patients with Subacute Stroke (PHYS-STROKE): a multicentre, randomised controlled, endpoint blinded trial" receives a QUEST NULL results award. This multicenter, randomized clinical trial tested the safety and efficacy of aerobic exercise on activities of daily living in the subacute phase after stroke. They found that the training did not improve maximal walking speed or activities of daily living compared with relaxation, while the rate of serious adverse events was higher in the aerobic exercise group. This article was published in 2019 in BMJ (DOI: 10.1136/bmj.l5101). Applicant: Alexander Nave, Klinik und Hochschulambulanz für Neurologie, Charité-Universitätsmedizin Berlin.
- The publication "Evaluating Modeling and Validation Strategies for Tooth Loss" receives a QUEST NULL results award. The authors tested prediction models for tooth loss under a large variety of modeling conditions and found that none of the developed models would be useful in a clinical setting, despite high accuracy. This article was published in 2019 in the Journal of Dental Research. (DOI: 10.1177/0022034519864889). Applicant: Falk Schwendicke, Department of Operative and Preventive Dentistry, Charité-Universitätsmedizin Berlin
- The publication "Cannabidivarin for the treatment of HIV-associated neuropathic pain – a randomized, blinded, controlled clinical trial" receives a QUEST NULL results award. This clinical trial tested Cannabidivarin as treatment for HIV-associated neuropathic pain and found that it was safe but failed to reduce neuropathic pain intensity in HIV-patients. This article was published in 2019 as a preprint in medRxiv (DOI: 10.1101/2019.12.20.19015495) Applicants: Luca Eibach and Prof. Chistoph Stein, Experimental Anaesthesiology, Charité Campus Benjamin Franklin, Charité - Universitätsmedizin Berlin.
- The publication "The Value of a Rapid Test of Human Regulatory T Cell Function Needs to be Revised" receives a QUEST replication study award. The article could demonstrate that a previously high-ranked published protocol for the assessment of human regulatory T cell (Treg) function is misleading and does not yield data of Treg function but rather of assay setup artifacts. This article was published in 2019 in the journal "Frontiers in Immunology" (DOI: 10.3389/fimmu.2019.00150). Applicant: Jacqueline Wendering, Institute for Medical Immunology, Charité - Universitätsmedizin Berlin
- The publication "Current Smoking Does Not Modify the Treatment Effect of Intravenous Thrombolysis in Acute Ischemic Stroke Patients—A Post-hoc Analysis of the WAKE-UP Trial" receives a QUEST NULL results award. The study could find no supporting evidence for the “smoking paradox”, which indicates that patients with acute ischemic stroke (AIS) who smoke at the time of their stroke may have a better prognosis after intravenous thrombolysis than non-smokers. This article was published in 2019 in the journal "frontiers in Neurology" (DOI: 10.3389/fneur.2019.01239). Applicant: Ludwig Schlemm, Department of Neurology with Experimental Neurology Campus Charité Mitte, Charité-Universitätsmedizin Berlin
- The publication "Bone structure determined by HR-MDCT does not correlate with micro-CT of lumbar vertebral biopsies: a prospective crosssectional human in vivo study" receives a QUEST NULL results award. The study tested if there is a correlation between the assessment of bone quality through preoperative lumbar high-resolution multidetector computed tomography (HR-MDCT) scans with the actual bone quality measured through microcomputed tomography (μCT) analysis of transpedicular vertebral body biopsies. The study found no significant correlations between the results of those two measurement methods. This article was published in 2020 in the Journal of Orthopaedic Surgery and Research (DOI: 10.1186/s13018-020-01895-0). Applicant: Yannick Palmowski, Center for Musculoskeletal Surgery, Charité - Universitätsmedizin Berlin
- The publication "Association Between Thrombus Perviousness Assessed on Computed Tomography and Stroke Cause" receives a QUEST Replication Study award. This study replicated a previous study on a new medical imaging biomarker for the detection of cardioembolic strokes, which was clinically relevant but which lacked a larger sample size and a multicentric design. This article was published in 2020 in the journal "Stroke" (DOI: 10.1161/STROKEAHA.120.031148). Applicant: Ludwig Schlemm, Department of Neurology with Experimental Neurology Campus Charité Mitte, Charité-Universitätsmedizin Berlin
- The publication "Focal brain ischemia in mice does not cause electrophysiological signs of critical illness neuropathy" receives a QUEST NULL results award. The study investigated in a mouse model if stroke lead to additional nerve or muscle damage. While an additional positive control showed the sensitivity of the used methods, no nerve or muscle damage was found. This article was published in 2020 in the journal "BMC Research Notes" (DOI: 10.1186/s13104-020-05248-2). Applicant: Wolfgang Böhmerle, Department of Neurology with Experimental Neurology Campus Charité Mitte, Charité-Universitätsmedizin Berlin
- The publication "No Impact of Functional Connectivity of the Motor System on the Resting Motor Threshold: A Replication Study" receives a QUEST Replication Study award. This study attempted to replicate the results of a previously published study on influencing factors on the resting motor threshold. Using a larger sample size than the previous study, they could not replicate this effect. This article was published in 2021 in the journal "frontiers in Neuroscience" (DOI: 10.3389/fnins.2021.627445). Applicant: Melina Engelhardt, Image Guidance Lab, Klinik für Neurochirurgie, Charité-Universitätsmedizin Berlin