Paper of the Month
FEBRUAR 2017 – Engineering an endocrine Neo-Pancreas by repopulation of a decellularized rat pancreas with islets of Langerhans
Die Auszeichnung BIH Paper of the Month ‘Februar’ ging an den BIH Charité Clinician Scientist Programmgeförderten Benjamin Strücker und sein Team.
Infobox
In der Open Access Publikation Engineering an endocrine Neo-Pancreas by repopulation of a decellularized rat pancreas with islets of Langerhans stellt Strücker eine Methode vor, mit der es möglich ist, nach Entfernung aller zellulären Bestandteile der Bauchspeicheldrüse der Ratte, die extrazelluläre Matrix mit Langerhansschen Inseln wieder zu besiedeln. In weiteren Tests konnten die Wissenschaftlerinnen und Wissenschaftler nachweisen, dass die Langerhansschen Inseln funktionsfähig waren und Insulin produzieren konnten.
H. Napierala, K.-H. Hillebrandt, N. Haep, P. Tang, M. Tintemann, J. Gassner, M. Noesser, H. Everwien, N. Seiffert, M. Kluge, E. Teegen, D. Polenz, S. Lippert, D. Geisel, A. Reutzel Selke, N. Raschzok, A. Andreou, J. Pratschke, I. M. Sauer & B. Struecker. Engineering an endocrine Neo-Pancreas by repopulation of a decellularized rat pancreas with islets of Langerhans. Scientific Reports 7. Article number: 41777 (2017) doi:10.1038/srep41777
Die Publikation steht hier zum Download zur Verfügung.
Abstract
Decellularization of pancreata and repopulation of these non-immunogenic matrices with islets and endothelial cells could provide transplantable, endocrine Neo- Pancreata. In this study, rat pancreata were perfusion decellularized and repopulated with intact islets, comparing three perfusion routes (Artery, Portal Vein, Pancreatic Duct). Decellularization effectively removed all cellular components but conserved the pancreas specific extracellular matrix. Digital subtraction angiography of the matrices showed a conserved integrity of the decellularized vascular system but a contrast emersion into the parenchyma via the decellularized pancreatic duct. Islets infused via the pancreatic duct leaked from the ductular system into the peri-ductular decellularized space despite their magnitude. TUNEL staining and Glucose stimulated insulin secretion revealed that islets were viable and functional after the process. We present the first available protocol for perfusion decellularization of rat pancreata via three different perfusion routes. Furthermore, we provide first proof-of-concept for the repopulation of the decellularized rat pancreata with functional islets of Langerhans. The presented technique can serve as a bioengineering platform to generate implantable and functional endocrine Neo-Pancreata.