In the BMBF-funded RECAST project, Prof. Birgit Sawitzki from the Institute for Medical Immunology at the Charité – Universitätsmedizin Berlin researches in collaboration with scientists from the Clinic for Pediatrics with Pneumology, Immunology and Intensive Care Medicine, the Medical Clinic with a focus on infectiology and pulmonology and the Institute of Virology describe the cellular and molecular mechanisms that trigger protective immune responses in children against SARS-CoV-2 and thus explain their resilience to severe COVID-19 courses. Initial studies indicate age-dependent differences in the reactions of the innate and adaptive immune systems. These differences will be examined in more detail in RECAST. For this purpose, samples from infected and non-infected people of various ages have already been collected for exploratory investigations at the transcript and protein level.
- Use of established CyTOF antibody panels & measurement of the collected blood samples
- Establishment of new antibody panels for measurements on the Spectral Analyzer (Cytek Aurora)
- Evaluation of the data in collaboration with the clinicians and bioinformaticians mentioned above
Prerequisite: completed doctorate in the field of immunology or cell biology; Knowledge of the establishment, execution and evaluation of cytometric analyzes, FloJo, GraphPad Prism
Please send your application documents as a PDF document (no more than 10 MB) to: Birgit.Sawitzki@charite.de