Leader Tatiana Borodina
tatiana.borodina@mdc-berlin.de
Phone: 030 9406 1341 | 030 450 643 367
Publications
We operate an impressive line of sequencing platforms (Illumina, PacBio, Oxford Nanopore), pipetting robots and analytical equipment at our high-throughput sequencing lab at the Rahel-Hirsch-Center. The team has extensive experience implementing novel protocols, automating library preparation, and methodological development.
Available services and technologies include:
- Consultations on NGS-related questions;
- Support in the experimental design of NGS projects;
- QC for DNA and RNA samples and sequencing libraries;
- Library preparation from bulk material for diverse -Seq applications:
- RNA sequencing (e.g. mRNA-, totalRNA-, 3’mRNA-, miRNA-Seq),
- DNA sequencing (e.g. whole genome, whole exome, amplicon, cfDNA),
- Epigenome (ChIP-Seq, Methyl-Seq, direct RNA and DNA sequencing);
- Short-read sequencing on Illumina instruments: iSeq, MiSeq, MiniSeq, NextSeq 2000, NovaSeq 6000, NovaSeq X plus;
- Long-read sequencing on Pacific Biosciences Revio;
- Long-read sequencing on Oxford Nanopore MinION and PromethION platforms
For consulting and assistance in planning of a sequencing experiment please contact us at seqteam@mdc-berlin.de or seqteam@bih-charite.de.
Actual pricelist valid for MDC, Charité and BIH users is available upon request. Large-scale projects require individual cost calculation.
If the service or the protocol you require is not in the pricelist, please contact us. Our official portfolio is limited to the most frequently requested services. We will find a customized solution for you.
Ordering and Turnaround time
Services are currently ordered per email to seqteam@mdc-berlin.de or to seqteam@bih-charite.de or to the members of the NGS Unit you are already in contact with.
Each service project has to be registered by filling the Project Registration Form and a relevant Sample Transfer Form – forms are sent out per email.
The specific number assigned by the NGS team to each project is used as a reference for further communication.
Generally, the projects are processed in the order of samples (libraries) receipt.
Scheduling of sequencing libraries preparation for any platform depends on the current workload and equipment availability.
Illumina sequencing schedule depends on how fast the samples requiring the same sequencing mode are collected to fill up a flowcell. If you're ready to sequencing libraries occupy the whole flowcell, usually they can be sequenced within 1-2 weeks after the delivery.
Loading of ready libraries on PacBio and Oxford Nanopore PromethION is also usually possible within 1-2 weeks, depending on the sequencing queue.
