hiPSC Biobank & Provision
The core provides users access to quality-controlled pluripotent stem cells. This ranges from reference hiPSC lines from healthy donors that are available cryopreserved and ready ‘in-culture’. In addition to the reference lines, users can request frozen vials of non-standard lines.
All lines are registered in hPSCreg and robustly validated and quality controlled. The lines were generated form healthy donor samples and produce high differentiation efficiencies in the number of various protocols. For the full portfolio of cell lines see tables below.
Reference hiPSC lines (healthy donor)
The reference lines are available to all researchers at BIH, Charité and MDC. Cells can be requested via openIRIS.
All external users are required to execute a Material Transfer Agreement (MTA) with us.
| Cell Line name | Gender | Cell type of origin | Reprogramming method | |
|---|---|---|---|---|
| BIHi001-B | male | Fibroblast Of Dermis | single-stranded RNA replicon vector | |
| BIHi004-B | female | Fibroblast Of Dermis | Episomal | |
| BIHi005-A | male | Fibroblast Of Dermis | mRNA | |
| BIHi250-A | female | Fibroblast Of Dermis | single-stranded RNA replicon vector |
Allen cell lines
The Allen Cell Catalog contains high quality, certified fluorescently tagged hiPSC lines that target key cellular structures and substructures.
CUSCO has filed an MTA that permits all researchers at BIH, Charité and MDC to work with the hiPSC lines of the Allen Cell Catalog provided through the core.
Currently available lines (quality controlled banks available)
| Line name | Tagged Gene | Structure | Fluorescent Tag |
|---|---|---|---|
| UCSFi001-A | untagged parental line | mone | none |
| UCSFi001-A-1 | Paxillin (PXN) | Matrix adhesions | EGFP |
| UCSFi001-A-8 | SEC61 translocon beta subunit (SEC61B) | Endoplasmic reticulum | mEGFP |
| UCSFi001-A-5 | translocase of outer mitochondrial membrane 20 (TOMM20) | mitochondria | mEGFP |
| UCSFi001-A-2 | tubulin-alpha 1b (TUBA 1B) | microtubules | mEGFP |
| UCSFi001-A-6 | lamin B1 (LMNB1) | Nuclear envelope | mEGFP |
| UCSFi001-A-9 | Fibrillarin (FBL) | nucleolus (dense fibrillar compontent) | mEGFP |
| UCSFi001-A-3 | actin beta (ACTB) | Actin filaments | mEGFP |
| UCSFi001-A-7 | Desmoplakin (DSP) | desmosomes | mEGFP |
| UCSFi001-A-13 | lysosomal associated membrane protein 1 (LAMP1) | lysosomes | mEGFP |
| UCSFi001-A-10 | tight junction protein 1 (TJP1) | Tight junctions | mEGFP |
| UCSFi001-A-11 | myosin heavy chain 10 (MYH10) | Actomyosin bundles | mEGFP |
| UCSFi001-A-14 | ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1) | Golgi | mEGFP |
| UCSFi001-A-15 | ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1) | Golgi | mEGFP |
| UCSFi001-A-18 | microtubule associated protein 1 light chain 3 beta (MAP1LC3B) | autophagosomes | mEGFP |
| UCSFi001-A-16 | tubulin-alpha 1b (TUBA1B) | microtubules | mTagRFP-T |
| UCSFi001-A-17 | centrin 2 (CETN2) | centrioles | mTagRFP-T |
| UCSFi001-A-19 | solute carrier family 25 member 17 (SLC25A17) | peroxisomes | mEGFP |
| UCSFi001-A-12 | safe harbor locus (AAVS1) | cytoplasm | mEGFP |
| UCSFi001-A-36 | safe harbor locus (AAVS1) | cytoplasm | mEGFP |
| UCSFi001-A-26 | troponin I1, slow skeletal type (TNNI1) | Sacromeric thin filaments | mEGFP |
| UCSFi001-A-20 | RAB5A, member RAS oncogene family (RAB5A) | endosomes | mEGFP |
| UCSFi001-A-21 | RAB5A, member RAS oncogene family (RAB5A) | endosomes | mEGFP |
| UCSFi001-A-30 | ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 (ATP2A2) | Sarcoplasmic reticulum/endoplasmic reticulum | mEGFP |
| UCSFi001-A-27 | Titin (TTN) | Sacromeres (M-line tag) | mEGFP |
| UCSFi001-A-29 | myosin light chain 7 (MYL7) | Sacromeres thick filaments | mEGFP |
| UCSFi001-A-22 | gap junction protein alpha 1 (GJA1) | Gap junctions | mEGFP |
| UCSFi001-A-23 | safe harbor locus (AAVS1) | Plasma membrane | mTagRFP-T |
| UCSFi001-A-24 | nucleophosmin 1 (NPM1) | Nucleolus (granular component) | mEGFP |
| UCSFi001-A-25 | catenin beta 1 (CTNNB1) | Adherens junctions | mEGFP |
| UCSFi001-A-33 | Sec61 translocon beta subunit / lamin B1 (SEC61B/LMNB1) | endoplasmic reticulum/ nuclear envelope | mEGFP / mTagRFP-T |
| UCSFi001-A-32 | myosin light chain 2 (MYL2) | Sacromeres thick filaments | mEGFP |
| UCSFi001-A-28 | histone cluster 1 H2B family member j (HIST1H2BJ) | histones | mEGFP |
| UCSFi001-A-34 | Dystrophin (DMD) | costameres | mEGFP |
| UCSFi001-A-31 | nucleoporin 153 (NUP153) | Nuclear pores | mEGFP |
| UCSFi001-A-37 | SRY-box transcription factor 2 (SOX2) | Transcription factor | mEGFP |
| UCSFi001-A-4 | actinin alpha 2 (ACTN2) | Sarcomeric z-disks | mEGFP |
| UCSFi001-A-35 | FUS RNA binding protein (FUS) | Paraspeckles / stress granules | mEGFP |
| UCSFi001-A-38 | fibrillarin / nucleophosmin 1 (FBL / NPM1) | Nucleolus (dense fibrillary component / granular component) | mEGFP / mTagRFP-T |
| UCSFi001-A-55 | translocase of outer mitochondrial membrane 20 / tubulin-alpha 1b (TOMM20 / TUBA1B) | Mitochondria / microtubules | mEGFP / mTagRFP-T |
Patient derived/disease specific hiPSC lines
CUSCO has maintains a growing portfolio of patient derived/disease specific hiPSC lines. If you are interested to work with these please get in contact with us.
| Line name | Disease | Gender | Cell type of origin |
|---|---|---|---|
| BIHi006-D | Focal segmental glomerulosclerosis (FSGS) | male | PBMC |
| BIHi007-A | Focal segmental glomerulosclerosis (FSGS) | male | PBMC |
| BIHi008-A | Focal segmental glomerulosclerosis (FSGS) | female | PBMC |
| BIHi009-A | Focal segmental glomerulosclerosis (FSGS) | male | PBMC |
| BIHi010-A | Focal segmental glomerulosclerosis (FSGS) | male | PBMC |
| BIHi011-A | Focal segmental glomerulosclerosis (FSGS) | female | PBMC |
| BIHi012-A | Focal segmental glomerulosclerosis (FSGS) | female | PBMC |
| BIHi015-A | Focal segmental glomerulosclerosis (FSGS) | male | PBMC |
| BIHi016-A | Focal segmental glomerulosclerosis (FSGS) | female | PBMC |
| BIHi017-A | Focal segmental glomerulosclerosis (FSGS) | male | PBMC |
| BIHi018-A | Focal segmental glomerulosclerosis (FSGS) | male | PBMC |
| BIHi019-A | Focal segmental glomerulosclerosis (FSGS) | male | PBMC |
| BIHi024-A | Focal segmental glomerulosclerosis (FSGS) | female | PBMC |
| BIHi025-A | Focal segmental glomerulosclerosis (FSGS) | male | PBMC |
| BIHi028-B | Focal segmental glomerulosclerosis (FSGS) | female | PBMC |
| BIHi029-A | Focal segmental glomerulosclerosis (FSGS) | male | PBMC |
| BIHi030-C | Focal segmental glomerulosclerosis (FSGS) | male | PBMC |
| BIHi031-B | Focal segmental glomerulosclerosis (FSGS) | female | PBMC |
| BIHi032-A | Focal segmental glomerulosclerosis (FSGS) | male | PBMC |
| BIHi038-B | Focal segmental glomerulosclerosis (FSGS) | female | PBMC |
| BCRTi001-A | fibrodysplasia ossificans progressive (FOP) | male | Urine |
| BCRTi002-A | fibrodysplasia ossificans progressive (FOP) | female | Urine |